Our Research
The cell membrane is a dynamic interface that controls material exchange, signalling and cellular responses. Its complex and asymmetric composition is actively organised by the cytoskeleton, lipid transporters, membrane contact sites and trafficking pathways.
We study how local changes in membrane composition and shape influence receptors, signalling and cell behaviour. Our work combines new membrane probes, high-resolution microscopy, protein conformational analysis and in vitro reconstitution.
We also investigate how cells generate specialised membrane domains through non-clathrin, non-dynamin-dependent endocytosis. Working across diverse model systems and disciplines, we aim to understand the cell membrane as an active living material.
Understanding mechanisms of endocytosis
We investigate how cells internalise and sort membrane components through clathrin-independent endocytic pathways. Using quantitative imaging, molecular perturbations and biophysical approaches, we study how mechanical forces, matrix cues, and the actin and microtubule cytoskeleton generate endocytic carriers, regulate cell-surface composition and influence signalling.
Plasma membrane organisation-
the living skin of a cell
We investigate how lipids and proteins are organised within the plasma membrane and how this organisation regulates cellular signalling and function. Our work explores how active forces generated by the cortical actin cytoskeleton create and remodel nanoscale membrane domains, particularly those containing GPI-anchored proteins. Using quantitative imaging, spectroscopy and biophysical approaches, we aim to understand the plasma membrane as an active, dynamic and responsive signalling platform.
Reconstructing the Active Cell Surface
We reconstruct simplified cell membranes from purified lipids and proteins to uncover the physical principles that organise the cell surface. By coupling fluid lipid bilayers to dynamic actin filaments and myosin motors, we examine how active forces remodel membrane components under precisely controlled conditions. These minimal systems allow us to test mechanistic models of membrane organisation and connect molecular interactions to the complex behaviour observed in living cells.
